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judy lieberman  (Addgene inc)


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    Addgene inc judy lieberman
    Judy Lieberman, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/judy+lieberman/pGL3-MYB-3'UTR+(Plasmid+%2325798)/pm38679332-234-34-36
    Average 93 stars, based on 5 article reviews
    judy lieberman - by Bioz Stars, 2026-11
    93/100 stars

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    Plasmid Preparation:

    Article Title: E-cadherin Interacts With Posttranslationally-Modified AGO2 to Enhance miRISC Activity.
    Article Snippet: .. Plasmids source: pCI-neo-RL-ZEB1 was a gift from Greg Goodall (Addgene plasmid # 35535) (Gregory et al., 2008); psiCHECK2-AURKB 3′ UTR was a gift from Judy Lieberman (Addgene plasmid #29475; Lal et al., 2009). ..

    Article Title: Cathepsin G Inhibition by Serpinb1 and Serpinb6 Prevents Programmed Necrosis in Neutrophils and Monocytes and Reduces GSDMD-Driven Inflammation.
    Article Snippet: Mature IL-1b in culture medium was measured by ELISA (eBioscience). .. pFlag-CMV-4 containing N-terminal Flag-tagged mouse GSDMD was a gift from Judy Lieberman (Addgene plasmid # 80950) (Liu et al., 2016). .. TNF-a, IL-6 and IL-1b in plasma and BAL were measured by ELISA (eBioscience).

    Article Title: Single chain variable fragment fused to maltose binding protein: a modular nanocarrier platform for the targeted delivery of antitumorals.
    Article Snippet: .. Expression. pACgp67B-HER2 plasmid containing the coding sequence for ML39 HER2 ScFv was a gift from Judy Lieberman (Addgene plasmid # 10794).27 ScFv coding sequence including a 3′-polyhistidine tail was amplified from pACgp67B-HER2 by PCR adding 5′BamHI and 3′HindIII restriction sites. .. A PCR fragment was sub-cloned in pMAL-TEV vector, a modified pMALc2X expression vector (New England Biolabs, Ipswich, MA, USA) where the factor Xa cleavage site has been substituted by a TEV protease cleavage site,28 to generate pMAL-TEV-ScFv HER2-His plasmid for the bacterial expression of a MBP-ScFv fusion protein.

    Article Title: Adipose tissue macrophage-derived microRNA-210-3p disrupts systemic insulin sensitivity by silencing GLUT4 in obesity.
    Article Snippet: .. For the Jo urn l P re- pro f 25 construction of the GLUT4 3’UTR-luciferase plasmid, we used pGL3-Myb-3’UTRluciferase vector (cat. no. #25798, Addgene) (43) as the backbone, which pGL3-MYB3'UTR was a gift from Judy Lieberman (Addgene plasmid # 25798 ; http://n2t.net/addgene:25798 ; RRID: Addgene_25798). .. We performed restriction digestion of the pGL3-Myb-3’UTR-luciferase vector using XbaI and SalI enzymes for the removal of the Myb-3’UTR region from the vector.

    Article Title: Down-regulation of the tumor suppressor miR-34a contributes to head and neck cancer by up-regulating the MET oncogene and modulating tumor immune evasion.
    Article Snippet: .. The following primary antibodies were used for this study: MET monoclonal antibody (3D4) (37–0100; Invitrogen); Ago2 Monoclonal Antibody (MA5–23515, Invitrogen); mouse IgG (sc-2025; Santa Cruz); FITC anti-STAT3 Phospho (Tyr705) (Biolegend); anti-mouse CD45 Antibody (30-F11, Biolegend); anti mouse MET antibody (ab51067, Abcam); anti-mouse CD11b Antibody (M1/70, Invitrogen); anti-mouse F4/80 Antibody (BM8; Biolegend); anti-mouse lineage cocktail (145-2C11; RB6-8C5; RA36B2; Ter-119; M1/70; Biolegend) and anti-mouse CD274 (PDL1) (10F.9G2; Biolegend), and anti-mouse Vimentin APC-conjugated Antibody (IC2105A, &D). hsa-miR-34a5p mimic and inhibitors were obtained from Ambion (Thermofisher). pLL3.7 was a gift from Luk Parijs (Addgene plasmid # 11795). pLL3.7 miR-34a-5p was a gift from Judy Lieberman (Addgene plasmid # 25791). .. The MET vector used in this study included both coding sequence and 3’UTR of MET and was constructed in a pBABE vector.

    Expressing:

    Article Title: Hepatitis B virus nucleocapsid uncoating: biological consequences and regulation by cellular nucleases.
    Article Snippet: .. Establishment of cell lines expressing wild-type and mutant TREX1 cDNA clones encoding full-length TREX1 and TREX1-D18N were gifts from Judy Lieberman (Addgene Cat. N. 27219 and 27220). ..

    Article Title: Single chain variable fragment fused to maltose binding protein: a modular nanocarrier platform for the targeted delivery of antitumorals.
    Article Snippet: .. Expression. pACgp67B-HER2 plasmid containing the coding sequence for ML39 HER2 ScFv was a gift from Judy Lieberman (Addgene plasmid # 10794).27 ScFv coding sequence including a 3′-polyhistidine tail was amplified from pACgp67B-HER2 by PCR adding 5′BamHI and 3′HindIII restriction sites. .. A PCR fragment was sub-cloned in pMAL-TEV vector, a modified pMALc2X expression vector (New England Biolabs, Ipswich, MA, USA) where the factor Xa cleavage site has been substituted by a TEV protease cleavage site,28 to generate pMAL-TEV-ScFv HER2-His plasmid for the bacterial expression of a MBP-ScFv fusion protein.

    Mutagenesis:

    Article Title: Hepatitis B virus nucleocapsid uncoating: biological consequences and regulation by cellular nucleases.
    Article Snippet: .. Establishment of cell lines expressing wild-type and mutant TREX1 cDNA clones encoding full-length TREX1 and TREX1-D18N were gifts from Judy Lieberman (Addgene Cat. N. 27219 and 27220). ..

    Clone Assay:

    Article Title: Hepatitis B virus nucleocapsid uncoating: biological consequences and regulation by cellular nucleases.
    Article Snippet: .. Establishment of cell lines expressing wild-type and mutant TREX1 cDNA clones encoding full-length TREX1 and TREX1-D18N were gifts from Judy Lieberman (Addgene Cat. N. 27219 and 27220). ..

    Sequencing:

    Article Title: Single chain variable fragment fused to maltose binding protein: a modular nanocarrier platform for the targeted delivery of antitumorals.
    Article Snippet: .. Expression. pACgp67B-HER2 plasmid containing the coding sequence for ML39 HER2 ScFv was a gift from Judy Lieberman (Addgene plasmid # 10794).27 ScFv coding sequence including a 3′-polyhistidine tail was amplified from pACgp67B-HER2 by PCR adding 5′BamHI and 3′HindIII restriction sites. .. A PCR fragment was sub-cloned in pMAL-TEV vector, a modified pMALc2X expression vector (New England Biolabs, Ipswich, MA, USA) where the factor Xa cleavage site has been substituted by a TEV protease cleavage site,28 to generate pMAL-TEV-ScFv HER2-His plasmid for the bacterial expression of a MBP-ScFv fusion protein.

    Amplification:

    Article Title: Single chain variable fragment fused to maltose binding protein: a modular nanocarrier platform for the targeted delivery of antitumorals.
    Article Snippet: .. Expression. pACgp67B-HER2 plasmid containing the coding sequence for ML39 HER2 ScFv was a gift from Judy Lieberman (Addgene plasmid # 10794).27 ScFv coding sequence including a 3′-polyhistidine tail was amplified from pACgp67B-HER2 by PCR adding 5′BamHI and 3′HindIII restriction sites. .. A PCR fragment was sub-cloned in pMAL-TEV vector, a modified pMALc2X expression vector (New England Biolabs, Ipswich, MA, USA) where the factor Xa cleavage site has been substituted by a TEV protease cleavage site,28 to generate pMAL-TEV-ScFv HER2-His plasmid for the bacterial expression of a MBP-ScFv fusion protein.

    Polymerase Chain Reaction:

    Article Title: Single chain variable fragment fused to maltose binding protein: a modular nanocarrier platform for the targeted delivery of antitumorals.
    Article Snippet: .. Expression. pACgp67B-HER2 plasmid containing the coding sequence for ML39 HER2 ScFv was a gift from Judy Lieberman (Addgene plasmid # 10794).27 ScFv coding sequence including a 3′-polyhistidine tail was amplified from pACgp67B-HER2 by PCR adding 5′BamHI and 3′HindIII restriction sites. .. A PCR fragment was sub-cloned in pMAL-TEV vector, a modified pMALc2X expression vector (New England Biolabs, Ipswich, MA, USA) where the factor Xa cleavage site has been substituted by a TEV protease cleavage site,28 to generate pMAL-TEV-ScFv HER2-His plasmid for the bacterial expression of a MBP-ScFv fusion protein.



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